Roaming is a reaction mechanism generating molecular products via wondering motions. The atomic movements during the roaming have never been directly observed. Here, we explore the atomic movements of a roaming-mediate isomerization using femtosecond X-ray liquidography.

Endoperoxidase NvfI catalyzes the incorporation of “three” oxygen atoms, including an endoperoxide and a hydroxyl group, into its substrate asnovolin A, in a single enzyme turnover reaction through a different mechanism from those of previously characterized endoperoxidases.

Introduction