Aqueous compartments bound by lipid membranes are increasingly used in drug delivery. The majority of these structures currently used in medicine are single compartment liposomes that release their contents slowly over a pre-determined length of time or suddenly upon a trigger such as temperature [1,2,3]. However, multi-compartment lipid membrane-based drug delivery systems have been developed for improved stability and drug retention. Composed of multiple non-concentric aqueous chambers surrounded by a network of lipid membranes, DepoFoam drug delivery particles are presently used to deliver the local analgesic bupivacaine [4,5]. Coalescence of compartments and permeation of drug through the membranes both play a role in the sustained release mechanism over time . However, the release cannot be stopped or adjusted once the particles have been injected into a patient.
As current lipid membrane-based drug delivery systems employ degradation of the membranes, the compartments are destroyed. In addition, they release only pre-existing contents and therefore cannot provide drugs that readily degrade. Lipid membrane-based delivery systems containing multiple compartments could carry out compartmentalized synthesis and delivery of drugs that would otherwise be prone to degradation. By inserting protein pores into the membranes, molecules could be shuttled through the compartments and externally released, while keeping the compartments intact. However, in such scenarios, pores insert into the lipid membranes with the external aqueous environment as well as the bilayers between the internal compartments, leading to uncontrolled leakage of molecules .
In search of a pore capable of controlled permeability, we turned to past work from the Hagan Bayley group. αHL-4H, a pore-forming toxin from Staphylococcus Aureus mutated to contain four specific histidine residues inside the barrel of the pore, was developed in 1997 as a metal ion sensor. Electrical recordings showed that this pore could be blocked with μM concentrations of Zn2+ and reopened by chelation with EDTA . Additionally, a similar variant was shown to controllably permeabilize mammalian cells , illustrating the biocompatible conditions for the use of these pores.
In this work, we demonstrated that small molecules could be selectively shuttled between internal compartments of a multicompartment delivery system and then controllably released, by cell-free expression of the αHL-4H protein within the system. We employed a two-compartment approach to activate a pro-drug analog and release it upon an environmental trigger, while keeping the compartmentalized structure intact. In these systems, the compartments had bilayers between each other as well as to an external aqueous environment . αHL-4H was synthesized in one compartment using cell-free synthesis machinery. The pore inserted into both the bilayer with the second compartment and the bilayer with the external aqueous environment. Pores to the external environment were blocked by μM concentrations of Zn2+ ions. However, the pores in the bilayer between compartments were open, allowing a small molecule pro-drug analog in the second compartment to move freely into the cell-free synthesis compartment, which also contained an enzyme that activated the small molecule. The product was held within the compartments until EDTA was added, to open the external pores. The activated molecule was then released and detected in the external solution.
Our approach eliminates the need to destroy the two-compartment structure. Additionally, the ability to hold the contents of the two compartments separately is important in the application of readily degradable drugs, high concentrations of drugs, or drugs that would otherwise inhibit cell-free protein synthesis. Control over the expression or insertion of pores could also be added to keep these contents separate until activation of the pro-drug is desired. The controlled release system from our study might also serve as a module to control the activity of large, patterned synthetic tissues, previously fabricated by our group [11,12]. In the future, controllable pores may enable the activation of multiple reactions within different compartments and release of their products separately, which would be impossible if the compartments were destroyed after the first release.
We have demonstrated the shuttling and subsequent enzymatic activation of a pro-drug analog within a multicompartment lipid-membrane delivery system, followed by controlled release of the product. The function of the system arises from the cell-free expression of a controllable membrane protein. Additionally, following release, both compartments are left intact. As the functionality developed in our study is based around the protein pore and not the specific structure, we envisage its application to control any lipid system.
Our full paper is available at:
1. Zylberberg, C. & Matosevic, S. Pharmaceutical liposomal drug delivery: a review of new delivery systems and a look at the regulatory landscape. Drug Deliv. 23, 3319-3329 (2016).
2. Pattni, B. S., Chupin, V. V. & Torchilin, V. P. New Developments in Liposomal Drug Delivery. Chem. Rev. 115, 10938-10966 (2015).
3. Bulbake, U., Doppalapudi, S., Ko, N. & Khan, W. Liposomal Formulations in Clinical Use: An Updated Review. Pharmaceutics 9, 12 (2017).
4. Pacira. DepoFoam. https://www.pacira.com/product...
5. Aratana. Nocita. https://nocita.aratana.com
6. Mantripragada, S. A lipid based depot (DepoFoam® technology) for sustained release drug delivery. Prog. Lipid Res. 41, 392-406 (2002).
7. Elani, Y., Law, R. V. & Ces, O. Vesicle-based artificial cells as chemical microreactors with spatially segregated reaction pathways. Nat. Commun. 5, 5305 (2014).
8. Braha, O., Walker, B., Cheley, S., Kasianowicz, J. J., Song, L., Gouaux, J. E. & Bayley, H. Designed pores as components for biosensors. Chem. Biol. 4, 497-505 (1997).
9. Russo, M. J., Bayley, H. & Toner, M. Reversible permeabilization of plasma membranes with an engineered switchable pore. Nat. Biotechnol. 15, 278-282 (1997).
10. Villar, G., Heron, A. J. & Bayley, H. Formation of droplet networks that function in aqueous environments. Nat. Nanotechnol. 6, 803-808 (2011).
11. Villar, G., Graham, A. D. & Bayley, H. A Tissue-Like Printed Material. Science 340, 48-52 (2013).
12. Booth, M. J., Restrepo Schild, V., Graham, A. D., Olof, S. N. & Bayley, H. Light-activated communication in synthetic tissues. Sci. Adv. 2, e1600056 (2016).
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